Introduction to Metabolic Reactions in Biological System

Preview 06:22

Glycolysis

Preview 16:44

Kreb Cycle

Preview 19:32

Anaerobic Oxidation

Preview 17:36

Introduction to Growth Media

Preview 09:42

Microbiology Medium Constituents

Preview 06:00

Microbiology Medium Preparation

Preview 13:01

Different Media for Eukaryotic Cells

Preview 05:13

Introduction to Microbial Growth Kinetics

Preview 07:53

Modes of Bacterial Cell Division

Preview 06:10

Measuring Microbial Growth

Preview 19:39

Stages in Normal Growth Curve

Preview 17:19

Introduction to Isolation of Gene Fragment

Preview 04:37

Different Approaches for Isolation of Gene of Interest

Preview 13:03

Construction of Genomic Library

Preview 05:02

Isolation of Genomic DNA

Preview 08:39

Generation of Suitable Size Fragments

Preview 05:53

Cloning into Suitable Vector

Preview 03:11

Transformation

Preview 02:59

Construction of cDNA Library

Preview 08:17

Methods of cDNA Synthesis

Preview 11:29

Introduction to Screening

Preview 08:52

Screening by DNA Hybridization

Preview 17:03

Screening by Immunological Methods

Preview 07:26

Screening by Enzymatic Methods

Preview 02:20

Isolation of Gene

Preview 06:17

Cell Division and Growth

Preview 04:01

DNA Replication and Synthesis

Preview 18:58

Polymerase Chain Reaction

Preview 12:44

DNA Primers

Preview 17:54

PCR Setup and Analysis

Preview 06:46

Introduction to Polymerase Chain Reaction (PCR)

Preview 03:23

Primer Designing Demo

Preview 19:28

PCR Setup and Analysis Demo

Preview 12:51

Application of PCR

Preview 10:01

Enzymes in Molecular Cloning

Preview 04:41

Restriction Methylase System

Preview 12:11

Types of Restriction Enzymes

Preview 09:20

Differences between Restriction Enzymes

Preview 06:41

Setting up Restriction Reactions

Preview 08:09

Ligase and Alkaline Phosphate

Preview 09:07

Additional tools for Facilitation of Cloning Reactions

Preview 07:06

Introduction to Vectors

Preview 13:20

Components of Vectors

Preview 05:11

Types of Vectors

Preview 13:56

Isolation of Plasmids

Preview 06:15

Isolation of Plasmids Demonstration

Preview 15:48

Eukaryotic Plasmids

Preview 17:17

Bacteriophage-Based Vectors

Preview 09:07

DNA Delivery in Host

Preview 11:00

Discovery and Mechanism of Transformation

Preview 08:06

Competent Cells

Preview 08:59

Transformation Steps

Preview 06:28

Transformation Demonstration

Preview 48:27

Electroporation

Preview 07:56

Factors Affecting Transformation Efficiency

Preview 03:40

Transformation in Yeast

Preview 07:22

DNA Delivery in Mammalian Cells

Preview 04:46

Chemical Transfection Methods

Preview 13:37

Liposome and Lipoplex Transfection Methods

Preview 05:46

Transfection Demonstration

Preview 19:40

Bactofection

Preview 06:34

Transduction

Preview 05:13

Introduction to Screening Recombinant Clones

Preview 04:37

Blue-White Screening

Preview 06:24

Antibiotic Sensitivity

Preview 04:13

Insertional Inactivation

Preview 03:41

Antibiotic Resistance Gene

Preview 05:40

Complementation of Mutation

Preview 10:17

Screening of Transfected Mammalian Cells

Preview 11:34

Confirmation of Cloned DNA

Preview 21:17

Introduction to Protein Production in Host

Preview 05:30

Protein Synthesis Machinery

Preview 15:06

Host as Production Machinery (E.Coli Expression System)

Preview 06:51

Promoters

Preview 19:22

Expression of Gene using E.Coli System

Preview 04:14

Induction of Protein Expression in Bacterial Cells

Preview 21:12

Factors Affecting Protein Synthesis in E.Coli

Preview 10:26

Expression of Fusion Protein in E.Coli

Preview 35:11

Yeast as an Expression System

Preview 17:33

Insect Cell Lines as Expression System

Preview 03:35

Generation and Screening of Recombinant Baculovirus

Preview 11:46

Mammalian Expression System

Preview 12:31

Transient Expression

Preview 07:22

Permanent Expression

Preview 10:10

Inducible Expression System

Preview 07:22